Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TSPYL2 / DENTT Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G110476-100
Catalog Number: LS-G110476
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DENTT(TSPYL2) (NM_022117), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CDA1 | CINAP | DENTT | CTCL | CTCL-associated antigen se20-4 | Cell division autoantigen 1 | HRIHFB2216 | Nuclear protein of 79 kDa | NP79 | TSPY-like protein 2 | SE20-4 | SE204 | TSPY-like 2 | CTCL tumor antigen se20-4 | TSPX | TSPYL2
- Origin Species
- Human
- NCBI GenBank
- NM_022117
- SwissProt
- Q9H2G4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 79.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDRPDEGPPA KTRRLSSSES PQRDPPPPPP PPPLLRLPLP PPQQRPRLQE ETEAAQVLAD61MRGVGLGPAL PPPPPYVILE EGGIRAYFTL GAECPGWDST IESGYGEAPP PTESLEALPT121PEASGGSLEI DFQVVQSSSF GGEGALETCS AVGWAPQRLV DPKSKEEAII IVEDEDEDER181ESMRSSRRRR RRRRRKQRKV KRESRERNAE RMESILQALE DIQLDLEAVN IKAGKAFLRL241KRKFIQMRRP FLERRDLIIQ HIPGFWVKAF LNHPRISILI NRRDEDIFRY LTNLQVQDLR301HISMGYKMKL YFQTNPYFTN MVIVKEFQRN RSGRLVSHST PIRWHRGQEP QARRHGNQDA361SHSFFSWFSN HSLPEADRIA EIIKNDLWVN PLRYYLRERG SRIKRKKQEM KKRKTRGRCE421VVIMEDAPDY YAVEDIFSEI SDIDETIHDI KISDFMETTD YFETTDNEIT DINENICDSE481NPDHNEVPNN ETTDNNESAD DHETTDNNES ADDNNENPED NNKNTDDNEE NPNNNENTYG541NNFFKGGFWG SHGNNQDSSD SDNEADEASD DEDNDGNEGD NEGSDDDGNE GDNEGSDDDD601RDIEYYEKVI EDFDKDQADY EDVIEIISDE SVEEEGIEEG IQQDEDIYEE GNYEEEGSED661VWEEGEDSDD SDLEDVLQVP NGWANPGKRG KTGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
693 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.