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TSPEAR Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TSPEAR Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TSPEAR antibodies →
$725.00each
Catalog Number: LS-G106845-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TSPEAR (NM_144991), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_144991

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
74.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSALLSLCFV LPLAAPGHGT QGWEPCTDLR PLDILAEVVP SDGATSGIRI VQVHGARGLQ
61LSVAAPRTMS FPASRIFSQC DLFPEEFSIV VTLRVPNLPP KRNEYLLTVV AEESDLLLLG
121LRLSPAQLHF LFLREDTAGA WQTRVSFRSP ALVDGRWHTL VLAVSAGVFS LTTDCGLPVD
181IMADVPFPAT LSVKGARFFV GSRRRAKGLF MGLVRQLVLL PGSDATPRLC PSRNAPLAVL
241SIPRVLQALT GKPEDNEVLK YPYETNIRVT LGPQPPCTEV EDAQFWFDAS RKGLYLCVGN
301EWVSVLAAKE RLDYVEEHQN LSTNSETLGI EVFRIPQVGL FVATANRKAT SAVYKWTEEK
361FVSYQNIPTH QAQAWRHFTI GKKIFLAVAN FEPDEKGQEF SVIYKWSHRK LKFTPYQSIA
421THSARDWEAF EVDGEHFLAV ANHREGDNHN IDSVIYKWNP ATRLFEANQT IATSGAYDWE
481FFSVGPYSFL VVANTFNGTS TKVHSHLYIR LLGSFQLFQS FPTFGAADWE VFQIGERIFL
541AVANSHSYDV EMQVQNDSYV INSVIYELNV TAQAFVKFQD ILTCSALDWE FFSVGEDYFL
601VVANSFDGRT FSVNSIIYRW QGYEGFVAVH SLPTVGCRDW EAFSTTAGAY LIYSSAKEPL
661SRVLRLRTR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

669 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.