Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TSPAN32 / PHEMX Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107034-100
Catalog Number: LS-G107034
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human PHEMX(TSPAN32) (NM_139022), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Tetraspanin 32 | Tetraspanin-32 | TSPAN32 | Pan-hematopoietic expression | PHEMX | TSSC6 | PHMX | Protein Phemx | Tspan-32 | Tumor-suppressing STF cDNA 6
- Origin Species
- Human
- NCBI GenBank
- AF176070
- SwissProt
- Q96QS1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 34.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGPWSRVRVA KCQMLVTCFF ILLLGLSVAT MVTLTYFGAH FAVIRRASLE KNPYQAVHQW61AFSAGLSLVG LLTLGAVLSA AATVREAQGL MAGGFLCFSL AFCAQVQVVF WRLHSPTQVE121DAMLDTYDLV YEQAMKGTSH VRRQELAAIQ DVFLCCGKKS PFSRLGSTEA DLCQGEEAAR181EDCLQGIRSF LRTHQQVASS LTSIGLALTV SALLFSSFLW FAIRCGCSLD RKGKYTLTPR241ACGRQPQEPS LLRCSQGGPT HCLHSEAVAI GPRGCSGSLR WLQESDAAPL PLSCHLAAHR301ALQGRSRGGL SGCPERGLSDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
320 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.