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TSKS Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TSKS Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G110614-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TSKS (NM_021733), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
STK22S1 | TSSKS | STK22 substrate 1 | TSKS | TSKS1
Origin Species
Human
NCBI GenBank
NM_021733
SwissProt
Q9UJT2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MASVVVKTIW QSKEIHEAGD TPTGVESCSQ LVPEAPRRVT SRAKGIPKKK KAVSFHGVEP
61QMSHQPMHWC LNLKRSSACT NVSLLNLAAM EPTDSTGTDS TVEDLSGQLT LAGPPASPTL
121PWDPDDADIT EILSGVNSGL VRAKDSITSL KEKTNRVNQH VQSLQSECSV LSENLERRRQ
181EAEELEGYCI QLKENCWKVT RSVEDAEIKT NVLKQNSALL EEKLRYLQQQ LQDETPRRQE
241AELQEPEEKQ EPEEKQEPEE KQKPEAGLSW NSLGPAATSQ GCPGPPGSPD KPSRPHGLVP
301AGWGMGPRAG EGPYVSEQEL QKLFTGIEEL RREVSSLTAR WHQEEGAVQE ALRLLGGLGG
361RVDGFLGQWE RAQREQAQTA RDLQELRGRA DELCTMVERS AVSVASLRSE LEGLGPLKPI
421LEEFGRQFQN SRRGPDLSMN LDRSHQGNCA RCASQGSQLS TESLQQLLDR ALTSLVDEVK
481QRGLTPACPS CQRLHKKILE LERQALAKHV RAEALSSTLR LAQDEALRAK NLLLTDKMKP
541EEKMATLDHL HLKMCSLHDH LSNLPLEGST GTMGGGSSAG TPPKQGGSAP EQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

592 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.