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TSHZ1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TSHZ1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G115736-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TSHZ1 (NM_005786), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CAA | NY-CO-33 | SDCCAG33 | Teashirt zinc finger 1 | TSH1 | Antigen NY-CO-33 | Teashirt family zinc finger 1 | Teashirt homolog 1 | TSHZ1
Origin Species
Human
NCBI GenBank
AF039698
SwissProt
Q6ZSZ6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
112.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MCNEETEIKE AQSYQNSPVS SATNQDAGYG SPFSESSDQL AHFKGSSSRE EKEDPQCPDS
61VSYPQDSLAQ IKAVYANLFS ESCWSSLALD LKKSGSTTST NDASQKESSA PTPTPPTCPV
121STTGPTTSTP STSCSSSTSH SSTTSTSSSS GYDWHQAALA KTLQQTSSYG LLPEPSLFST
181VQLYRQNNKL YGSVFTGASK FRCKDCSAAY DTLVELTVHM NETGHYRDDN RDKDSEKTKR
241WSKPRKRSLM EMEGKEDAQK VLKCMYCGHS FESLQDLSVH MIKTKHYQKV PLKEPVPAIT
301KLVPSTKKRA LQDLAPPCSP EPAGMAAEVA LSESAKDQKA ANPYVTPNNR YGYQNGASYT
361WQFEARKAQI LKCMECGSSH DTLQQLTAHM MVTGHFLKVT TSASKKGKQL VLDPVVEEKI
421QSIPLPPTTH TRLPASSIKK QPDSPAGSTT SEEKKEPEKE KPPVAGDAEK IKEESEDSLE
481KFEPSTLYPY LREEDLDDSP KGGLDILKSL ENTVSTAISK AQNGAPSWGG YPSIHAAYQL
541PGTVKPLPAA VQSVQVQPSY AGGVKSLSSA EHNALLHSPG SLTPPPHKSN VSAMEELVEK
601VTGKVNIKKE ERPPEKEKSS LAKAASPIAK ENKDFPKTEE VSGKPQKKGP EAETGKAKKE
661GPLDVHTPNG TEPLKAKVTN GCNNLGIIMD HSPEPSFINP LSALQSIMNT HLGKVSKPVS
721PSLDPLAMLY KISNSMLDKP VYPATPVKQA DAIDRYYYEN SDQPIDLTKS KNKPLVSSVA
781DSVASPLRES ALMDISDMVK NLTGRLTPKS STPSTVSEKS DADGSSFEEA LDELSPVHKR
841KGRQSNWNPQ HLLILQAQFA SSLRETTEGK YIMSDLGPQE RVHISKFTGL SMTTISHWLA
901NVKYQLRRTG GTKFLKNLDT GHPVFFCNDC ASQFRTASTY ISHLETHLGF SLKDLSKLPL
961NQIQEQQNVS KVLTNKTLGP LGATEEDLGS TFQCKLCNRT FASKHAVKLH LSKTHGKSPE
1021DHLIYVTELE KQ
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1032 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.