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TSGA10IP Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TSGA10IP Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TSGA10IP antibodies →
$725.00each
Catalog Number: LS-G106039-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TSGA10IP (NM_152762), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
FAM161C | Tsga10-interacting protein | TSGA10IP
Origin Species
Human
NCBI GenBank
NM_152762
SwissProt
Q3SY00

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGQDTDMLNT YQQLVRTPSV RPGQDVRLQA PGTRTGLLKL LSTVSQDKQG CLGSGDGVPN
61QDLQQRSQSS RQTAKKDRKP RGQSKKGQGS EESEDHFPLL PRKPSFPFQW AWESIATDVR
121AVLQPSSPTP GHQALPMPSS FSQRQSRRKS TANLPEAHGC CWKTEAQNLK ARQQLGAWGG
181VSIPTGKGEL GSEPPSGLQL PGRRPGSGSA SDKQVQLQSL GAEEAERGLS SGVLPQRPRR
241GSISEEEQFS EATEEAEEGE HRTPCRRRAG CQRKGQISGE EASDEGEVQG QSQGSSPSFN
301NLRRRQWRKT RAKELQGPWD LEKLHRQLQR DLDCGPQKLP WKTLRAAFQA SKRNGKAYAS
361GYDETFVSAN LPNRTFHKRQ EATRSLLQAW ERQRQEERQQ AELRRARTQH VQRQVAHCLA
421AYAPRGSRGP GAAQRKLEEL RRQERQRFAE YQAELQGIQH RVQARPFLFQ QAMQANARLT
481VTRRFSQVLS ALGLDEEQLL SEAGKVDREG TPRKPRSHRS VGVRMEHSPQ RPPRTEPTGS
541QPDRHYNPSL DPECSP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

556 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.