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TSGA10 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TSGA10 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G109497-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TSGA10 (NM_025244), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Testis specific | 10 | TSGA10 | Cancer/testis antigen 79 | CEP4L | CT79
Origin Species
Human
NCBI GenBank
NM_025244
SwissProt
Q9BZW7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
81.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMRSRSKSPR RPSPTARGAN CDVELLKTTT RDREELKCML EKYERHLAEI QGNVKVLKSE
61RDKIFLLYEQ AQEEITRLRR EMMKSCKSPK STTAHAILRR VETERDVAFT DLRRMTTERD
121SLRERLKIAQ ETAFNEKAHL EQRIEELECT VHNLDDERME QMSNMTLMKE TISTVEKEMK
181SLARKAMDTE SELGRQKAEN NSLRLLYENT EKDLSDTQRH LAKKKYELQL TQEKIMCLDE
241KIDNFTRQNI AQREEISILG GTLNDLAKEK ECMQACLDKK SENIASLGES LAMKEKTISG
301MKNIIAEMEQ ASRQCTEALI VCEQDVSRMR RQLDETNDEL AQIARERDIL AHDNDNLQEQ
361FAKAKQENQA LSKKLNDTHN ELNDIKQKVQ DTNLEVNKLK NILKSEESEN RQMMEQLRKA
421NEDAENWENK ARQSEADNNT LKLELITAEA EGNRLKEKVD SLNREVEQHL NAERSYKSQI
481STLHKSVVKM EEELQKVQFE KVSALADLSS TRELCIKLDS SKELLNRQLV AKDQEIEMRE
541NELDSAHSEI ELLRSQMANE RISMQNLEAL LVANRDKEYQ SQIALQEKES EIQLLKEHLC
601LAENKMAIQS RDVAQFRNVV TQLEADLDIT KRQLGTERFE RERAVQELRR QNYSSNAYHM
661SSTMKPNTKC HSPERAHHRS PDRGLDRSLE ENLCYRDF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

698 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.