Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TSC22D3 / GILZ Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116889-100
Catalog Number: LS-G116889
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TSC22D3(TSC22D3) (NM_004089), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DSIPI | DIP | DSIP-immunoreactive peptide | GILZ | TSC22D3 | TSC-22 related protein | Protein DIP | TSC22 domain family protein 3 | HDIP | TSC-22-like protein | TSC-22-related protein | TSC-22R | TSC22 domain family | member 3
- Origin Species
- Human
- NCBI GenBank
- NM_004089
- SwissProt
- Q99576
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 14.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNTEMYQTPM EVAVYQLHNF SISFFSSLLG GDVVSVKLDN SASGASVVAI DNKIEQAMDL61VKNHLMYAVR EEVEILKEQI RELVEKNSQL ERENTLLKTL ASPEQLEKFQ SCLSPEEPAP121ESPQVPEAPG GSAVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
134 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.