Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TS / Thromboxane Synthase Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G119357-100
Catalog Number: LS-G119357
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TBXAS1 (NM_001061), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- BDPLT14 | CYP5 | CYP5A1 | Cytochrome P450 5A1 | GHOSAL | Thromboxane Synthase | TBXAS1 | Thromboxane-A synthase | TS | TXAS | TXS | THAS | TXA synthase
- Origin Species
- Human
- NCBI GenBank
- NM_001061
- SwissProt
- P24557
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 60.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MMEALGFLKL EVNGPMVTVA LSVALLALLK WYSTSAFSRL EKLGLRHPKP SPFIGNLTFF61RQGFWESQME LRKLYGPLCG YYLGRRMFIV ISEPDMIKQV LVENFSNFTN RMASGLEFKS121VADSVLFLRD KRWEEVRGAL MSAFSPEKLN EMVPLISQAC DLLLAHLKRY AESGDAFDIQ181RCYCNYTTDV VASVAFGTPV DSWQAPEDPF VKHCKRFFEF CIPRPILVLL LSFPSIMVPL241ARILPNKNRD ELNGFFNKLI RNVIALRDQQ AAEERRRDFL QMVLDARHSA SPMGVQDFDI301VRDVFSSTGC KPNPSRQHQP SPMARPLTVD EIVGQAFIFL IAGYEIITNT LSFATYLLAT361NPDCQEKLLR EVDVFKEKHM APEFCSLEEG LPYLDMVIAE TLRMYPPAFR FTREAAQDCE421VLGQRIPAGA VLEMAVGALH HDPEHWPSPE TFNPERFTAE ARQQHRPFTY LPFGAGPRSC481LGVRLGLLEV KLTLLHVLHK FRFQACPETQ VPLQLESKSA LGPKNGVYIK IVSRTRTRPLEQKLISEEDLAANDILDYKDDDDKV
534 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.