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TRUB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRUB2 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113078-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRUB2 (NM_015679), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CLONE24922 | TRUB2 | RP11-339B21.1
Origin Species
Human
NCBI GenBank
NM_015679
SwissProt
O95900

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
36.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGSAGLSRLH GLFAVYKPPG LKWKHLRDTV ELQLLKGLNA RKPPAPKQRV RFLLGPMEGS
61EEKELTLTAT SVPSFINHPL VCGPAFAHLK VGVGHRLDAQ ASGVLVLGVG HGCRLLTDMY
121NAHLTKDYTV RGLLGKATDD FREDGRLVEK TTYDHVTREK LDRILAVIQG SHQKALVMYS
181NLDLKTQEAY EMAVRGLIRP MNKSPMLITG IRCLYFAPPE FLLEVQCMHE TQKELRKLVH
241EIGLELKTTA VCTQVRRTRD GFFTLDSALL RTQWDLTNIQ DAIRAATPQV AAELEKSLSP
301GLDTKQLPSP GWSWDSQGPS STLGLERGAG QTRTRPLEQK LISEEDLAAN DILDYKDDDD
361KV

362 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.