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TRPS1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRPS1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TRPS1 antibodies →
$725.00each
Catalog Number: LS-G114150-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRPS1 (NM_014112), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GC79 | LGCR | TRPS1 | Zinc finger protein GC79
Origin Species
Human
NCBI GenBank
NM_014112
SwissProt
Q9UHF7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
142.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPYEVNAGYD FTNMVRKKNP PLRNVASEGE GQILEPIGTE SKVSGKNKEF SADQMSENTD
61QSDAAELNHK EEHSLHVQDP SSSSKKDLKS AVLSEKAGFN YESPSKGGNF PSFPHDEVTD
121RNMLAFSSPA AGGVCEPLKS PQRAEADDPQ DMACTPSGDS LETKEDQKMS PKATEETGQA
181QSGQANCQGL SPVSVASKNP QVPSDGGVRL NKSKTDLLVN DNPDPAPLSP ELQDFKCNIC
241GYGYYGNDPT DLIKHFRKYH LGLHNRTRQD AELDSKILAL HNMVQFSHSK DFQKVNRSVF
301SGVLQDINSS RPVLLNGTYD VQVTSGGTFI GIGRKTPDCQ GNTKYFRCKF CNFTYMGNSS
361TELEQHFLQT HPNKIKASLP SSEVAKPSEK NSNKSIPALQ SSDSGDLGKW QDKITVKAGD
421DTPVGYSVPI KPLDSSRQNG TEATSYYWCK FCSFSCESSS SLKLLEHYGK QHGAVQSGGL
481NPELNDKLSR GSVINQNDLA KSSEGETMTK TDKSSSGAKK KDFSSKGAED NMVTSYNCQF
541CDFRYSKSHG PDVIVVGPLL RHYQQLHNIH KCTIKHCPFC PRGLCSPEKH LGEITYPFAC
601RKSNCSHCAL LLLHLSPGAA GSSRVKHQCH QCSFTTPDVD VLLFHYESVH ESQASDVKQE
661ANHLQGSDGQ QSVKESKEHS CTKCDFITQV EEEISRHYRR AHSCYKCRQC SFTAADTQSL
721LEHFNTVHCQ EQDITTANGE EDGHAISTIK EEPKIDFRVY NLLTPDSKMG EPVSESVVKR
781EKLEEKDGLK EKVWTESSSD DLRNVTWRGA DILRGSPSYT QASLGLLTPV SGTQEQTKTL
841RDSPNVEAAH LARPIYGLAV ETKGFLQGAP AGGEKSGALP QQYPASGENK SKDESQSLLR
901RRRGSGVFCA NCLTTKTSLW RKNANGGYVC NACGLYQKLH STPRPLNIIK QNNGEQIIRR
961RTRKRLNPEA LQAEQLNKQQ RGSNEEQVNG SPLERRSEDH LTESHQREIP LPSLSKYEAQ
1021GSLTKSHSAQ QPVLVSQTLD IHKRMQPLHI QIKSPQESTG DPGNSSSVSE GKGSSERGSP
1081IEKYMRPAKH PNYSPPGSPI EKYQYPLFGL PFVHNDFQSE ADWLRFWSKY KLSVPGNPHY
1141LSHVPGLPNP CQNYVPYPTF NLPPHFSAVG SDNDIPLDLA IKHSRPGPTA NGASKEKTKA
1201PPNVKNEGPL NVVKTEKVDR STQDELSTKC VHCGIVFLDE VMYALHMSCH GDSGPFQCSI
1261CQHLCTDKYD FTTHIQRGLH RNNAQVEKNG KPKE
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

1294 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.