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TRPC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRPC3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G100024-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRPC3 (NM_003305), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HTrp-3 | HTrp3 | TRP3 | TRPC3 | Transient receptor protein 3 | TRP-3 | Trrp3
Origin Species
Human
NCBI GenBank
NM_003305
SwissProt
Q13507

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
97.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MEGSPSLRRM TVMREKGRRQ AVRGPAFMFN DRGTSLTAEE ERFLDAAEYG NIPVVRKMLE
61ESKTLNVNCV DYMGQNALQL AVGNEHLEVT ELLLKKENLA RIGDALLLAI SKGYVRIVEA
121ILNHPGFAAS KRLTLSPCEQ ELQDDDFYAY DEDGTRFSPD ITPIILAAHC QKYEVVHMLL
181MKGARIERPH DYFCKCGDCM EKQRHDSFSH SRSRINAYKG LASPAYLSLS SEDPVLTALE
241LSNELAKLAN IEKEFKNDYR KLSMQCKDFV VGVLDLCRDS EEVEAILNGD LESAEPLEVH
301RHKASLSRVK LAIKYEVKKF VAHPNCQQQL LTIWYENLSG LREQTIAIKC LVVLVVALGL
361PFLAIGYWIA PCSRLGKILR SPFMKFVAHA ASFIIFLGLL VFNASDRFEG ITTLPNITVT
421DYPKQIFRVK TTQFTWTEML IMVWVLGMMW SECKELWLEG PREYILQLWN VLDFGMLSIF
481IAAFTARFLA FLQATKAQQY VDSYVQESDL SEVTLPPEIQ YFTYARDKWL PSDPQIISEG
541LYAIAVVLSF SRIAYILPAN ESFGPLQISL GRTVKDIFKF MVLFIMVFFA FMIGMFILYS
601YYLGAKVNAA FTTVEESFKT LFWSIFGLSE VTSVVLKYDH KFIENIGYVL YGIYNVTMVV
661VLLNMLIAMI NSSYQEIEDD SDVEWKFARS KLWLSYFDDG KTLPPPFSLV PSPKSFVYFI
721MRIVNFPKCR RRRLQKDIEM GMGNSKSRLN LFTQSNSRVF ESHSFNSILN QPTRYQQIMK
781RLIKRYVLKA QVDKENDEVN EGELKEIKQD ISSLRYELLE DKSQATEELA ILIHKLSEKL
841NPSMLRCE
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

848 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.