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TRPC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRPC1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TRPC1 antibodies →
$725.00each
Catalog Number: LS-G117434-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRPC1 (TRPC1) (NM_003304), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HTRP-1 | TRP1 | TRP-1 | TRPC1 | Trrp1 | Transient receptor protein 1
Origin Species
Human
NCBI GenBank
NM_003304
SwissProt
P48995

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
87.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MMAALYPSTD LSGASSSSLP SSPSSSSPNE VMALKDVREV KEENTLNEKL FLLACDKGDY
61YMVKKILEEN SSGDLNINCV DVLGRNAVTI TIENENLDIL QLLLDYGCQK LMERIQNPEY
121STTMDVAPVI LAAHRNNYEI LTMLLKQDVS LPKPHAVGCE CTLCSAKNKK DSLRHSRFRL
181DIYRCLASPA LIMLTEEDPI LRAFELSADL KELSLVEVEF RNDYEELARQ CKMFAKDLLA
241QARNSRELEV ILNHTSSDEP LDKRGLLEER MNLSRLKLAI KYNQKEFVSQ SNCQQFLNTV
301WFGQMSGYRR KPTCKKIMTV LTVGIFWPVL SLCYLIAPKS QFGRIIHTPF MKFIIHGASY
361FTFLLLLNLY SLVYNEDKKN TMGPALERID YLLILWIIGM IWSDIKRLWY EGLEDFLEES
421RNQLSFVMNS LYLATFALKV VAHNKFHDFA DRKDWDAFHP TLVAEGLFAF ANVLSYLRLF
481FMYTTSSILG PLQISMGQML QDFGKFLGMF LLVLFSFTIG LTQLYDKGYT SKEQKDCVGI
541FCEQQSNDTF HSFIGTCFAL FWYIFSLAHV AIFVTRFSYG EELQSFVGAV IVGTYNVVVV
601IVLTKLLVAM LHKSFQLIAN HEDKEWKFAR AKLWLSYFDD KCTLPPPFNI IPSPKTICYM
661ISSLSKWICS HTSKGKVKRQ NSLKEWRNLK QKRDENYQKV MCCLVHRYLT SMRQKMQSTD
721QATVENLNEL RQDLSKFRNE IRDLLGFRTS KYAMFYPRN
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

759 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.