Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TRNT1 / CCA1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112918-100
Catalog Number: LS-G112918
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRNT1 (NM_016000), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- CCA1 | CGI-47 | Mt tRNA adenylyltransferase | MtCCA | Mt CCA-adding enzyme | Mt tRNA CCA-diphosphorylase | Mt tRNA CCA-pyrophosphorylase | TRNT1
- Origin Species
- Human
- NCBI GenBank
- NM_016000
- SwissProt
- Q96Q11
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKLQSPEFQS LFTEGLKSLT ELFVKENHEL RIAGGAVRDL LNGVKPQDID FATTATPTQM61KEMFQSAGIR MINNRGEKHG TITARLHEEN FEITTLRIDV TTDGRHAEVE FTTDWQKDAE121RRDLTINSMF LGFDGTLFDY FNGYEDLKNK KVRFVGHAKQ RIQEDYLRIL RYFRFYGRIV181DKPGDHDPET LEAIAENAKG LAGISGERIW VELKKILVGN HVNHLIHLIY DLDVAPYIGL241PANASLEEFD KVSKNVDGFS PKPVTLLASL FKVQDDVTKL DLRLKIAKEE KNLGLFIVKN301RKDLIKATDS SDPLKPYQDF IIDSREPDAT TRVCELLKYQ GEHCLLKEMQ QWSIPPFPVS361GHDIRKVGIS SGKEIGALLQ QLREQWKKSG YQMEKDELLS YIKKTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
405 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.