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TRMT61B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRMT61B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TRMT61B antibodies →
$504.00each
Catalog Number: LS-G112131-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRMT61B (NM_017910), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TRMT61B
Origin Species
Human
NCBI GenBank
NM_017910
SwissProt
Q9BVS5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLMAWCRGPV LLCLRQGLGT NSFLHGLGQE PFEGARSLCC RSSPRDLRDG EREHEAAQRK
61APGAESCPSL PLSISDIGTG CLSSLENLRL PTLREESSPR ELEDSSGDQG RCGPTHQGSE
121DPSMLSQAQS AIEVEERHVS PSCSTSRERP FQAGELILAE TGEGETKFKK LFRLNNFGLL
181NSNWGAVPFG KIVGKFPGQI LRSSFGKQYM LRRPALEDYV VLMKRGTAIT FPKDINMILS
241MMDINPGDTV LEAGSGSGGM SLFLSKAVGS QGRVISFEVR KDHHDLAKKN YKHWRDSWKL
301SHVEEWPDNV DFIHKDISGA TEDIKSLTFD AVALDMLNPH VTLPVFYPHL KHGGVCAVYV
361VNITQVIELL DGIRTCELAL SCEKISEVIV RDWLVCLAKQ KNGILAQKVE SKINTDVQLD
421SQEKIGVKGE LFQEDDHEES HSDFPYGSFP YVARPVHWQP GHTAFLVKLR KVKPQLNTRT
481RPLEQKLISE EDLAANDILD YKDDDDKV

508 aa

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.