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TRMT2B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRMT2B Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G129855-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRMT2B (NM_001167971), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
CXorf34 | DJ341D10.3 | TRM2 homolog | TRMT2B
Origin Species
Human
NCBI GenBank
NM_024917
SwissProt
Q96GJ1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
51.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAGLKRRVPL HSLRYFISMV GLFSKPGLLP WYARNPPGWS QLFLGTVCKG DFTRVIATKC
61QKGQKSQKKP SHLGPLDGSW QERLADVVTP LWRLSYEEQL KPVINGYRNK STFSVNRGPD
121GNPKTVGFYL GTWRDGNVVC VQSNHLKNIP EKHSQVAQYY EVFLRQSPLE PCLVFHEGGY
181WRELTVRTNS QGHTMAIITF HPQKLSQEEL HVQKEIVKEF FIRGPGAACG LTSLYFQEST
241MTRCSHQQSP YQLLFGEPYI FEELLSLKIR ISPDAFFQIN TAGAEMLYRT VGELTGVNSD
301TILLDICCGT GVIGLSLAQH TSRVLGIELL EQAVEDARWT AAFNGITNSE FHTGQAEKIL
361PGLLKSKEDG QSIVAVVNPA RAGLHYKVIQ AIRNFRAIHT LVFVSCKLHG ESTRNVIELC
421CPPDPAKKLL GEPFVLQQAV PVDLFPHTPH CELVLLFTR
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

459 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.