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TRMT2A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRMT2A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G110281-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human HTF9C(TRMT2A) (NM_022727), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TRMT2A | HTF9C
Origin Species
Human
NCBI GenBank
NM_022727
SwissProt
Q8IZ69

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSENLDNEGP KPMESCGQES SSALSCPTVS VPPAAPAALE EVEKEGAGAA TGPGPQPGLY
61SYIRDDLFTS EIFKLELQNV PRHASFSDVR RFLGRFGLQP HKTKLFGQPP CAFVTFRSAA
121ERDKALRVLH GALWKGRPLS VRLARPKADP MARRRRQEGE SEPPVTRVAD VVTPLWTVPY
181AEQLERKQLE CEQVLQKLAK EIGSTNRALL PWLLEQRHKH NKACCPLEGV RPSPQQTEYR
241NKCEFLVGVG VDGEDNTVGC RLGKYKGGTC AVAAPFDTVH IPEATKQVVK AFQEFIRSTP
301YSAYDPETYT GHWKQLTVRT SRRHQAMAIA YFHPQKLSPE ELAELKTSLA QHFTAGPGRA
361SGVTCLYFVE EGQRKTPSQE GLPLEHVAGD RCIHEDLLGL TFRISPHAFF QVNTPAAEVL
421YTVIQDWAQL DAGSMVLDVC CGTGTIGLAL ARKVKRVIGV ELCPEAVEDA RVNAQDNELS
481NVEFHCGRAE DLVPTLVSRL ASQHLVAILD PPRAGLHSKV ILAIRRAKNL RRLLYVSCNP
541RAAMGNFVDL CRAPSNRVKG IPFRPVKAVA VDLFPQTPHC EMLILFERVE HPNGTGVLGP
601HSPPAQPTPG PPDNTLQETG TFPSS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

625 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.