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TRMT1L Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRMT1L Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TRMT1L antibodies →
$504.00each
Catalog Number: LS-G109342-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C1orf25(TRMT1L) (NM_030934), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
BG120K12.3 | C1orf25 | MST070 | MSTP070 | TRM1L | TRMT1-like protein | TRMT1L | TRM1-like protein
Origin Species
Human
NCBI GenBank
NM_030934
SwissProt
Q7Z2T5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
81.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MENMAEEELL PLEKEEVEVA QVQVPTPARD SAGVPAPAPD SALDSAPTPA SAPAPAPALA
61QAPALSPSLA SAPEEAKSKR HISIQRQLAD LENLAFVTDG NFDSASSLNS DNLDAGNRQA
121CPLCPKEKFR ACNSHKLRRH LQNLHWKVSV EFEGYRMCIC HLPCRPVKPN IIGEQITSKM
181GAHYHCIICS ATITRRTDML GHVRRHMNKG ETKSSYIAAS TAKPPKEILK EADTDVQVCP
241NYSIPQKTDS YFNPKMKLNR QLIFCTLAAL AEERKPLECL DAFGATGIMG LQWAKHLGNA
301VKVTINDLNE NSVTLIQENC HLNKLKVVVD SKEKEKSDDI LEEGEKNLGN IKVTKMDANV
361LMHLRSFDFI HLDPFGTSVN YLDSAFRNIR NLGIVSVTST DISSLYAKAQ HVARRHYGCN
421IVRTEYYKEL AARIVVAAVA RAAARCNKGI EVLFAVALEH FVLVVVRVLR GPTSADETAK
481KIQYLIHCQW CEERIFQKDG NMVEENPYRQ LPCNCHGSMP GKTAIELGPL WSSSLFNTGF
541LKRMLFESLH HGLDDIQTLI KTLIFESECT PQSQFSIHAS SNVNKQEENG VFIKTTDDTT
601TDNYIAQGKR KSNEMITNLG KKQKTDVSTE HPPFYYNIHR HSIKGMNMPK LKKFLCYLSQ
661AGFRVSRTHF DPMGVRTDAP LMQFKSILLK YSTPTYTGGQ SESHVQSASE DTVTERVEMS
721VNDKAEASGC RRW
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

733 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.