Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TRMT10C / RG9MTD1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112203-100
Catalog Number: LS-G112203
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RG9MTD1(TRMT10C) (NM_017819), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MRPP1 | HNYA | TRMT10C | NY-REN-49 antigen | RG9MTD1
- Origin Species
- Human
- NCBI GenBank
- NM_017819
- SwissProt
- Q7L0Y3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 47.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSVSVNFFRP FTRFLVPFTL HRKRNNLTIL QRYMSSKIPA VTYPKNESTP PSEELELDKW61KTTMKSSVQE ECVSTISSSK DEDPLAATRE FIEMWRLLGR EVPEHITEEE LKTLMECVSN121TAKKKYLKYL YTKEKVKKAR QIKKEMKAAA REEAKNIKLL ETTEEDKQKN FLFLRLWDRN181MDIAMGWKGA QAMQFGQPLV FDMAYENYMK RKELQNTVSQ LLESEGWNRR NVDPFHIYFC241NLKIDGALHR ELVKRYQEKW DKLLLTSTEK SHVDLFPKDS IIYLTADSPN VMTTFRHDKV301YVIGSFVDKS MQPGTSLAKA KRLNLATECL PLDKYLQWEI GNKNLTLDQM IRILLCLKNN361GNWQEALQFV PKRKHTGFLE ISQHSQEFIN RLKKAKTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
397 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.