Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TRMO / C9orf156 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G112630-100
Catalog Number: LS-G112630
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C9orf156(TRMO) (NM_016481), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C9orf156 | NAP1 | Nef associated protein 1 | Nef-associated protein 1 | HSPC219 | Thioesterase NAP1 | RP11-23B15.3
- Origin Species
- Human
- NCBI GenBank
- NM_016481
- SwissProt
- Q9BU70
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 48.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MRGLEEPGPR PTATPCGCVK PALETGNLLT EPVGYLESCF SAKNGTPRQP SICSYSRACL61RIRKRIFNNP EHSLMGLEQF SHVWILFVFH KNGHLSCKAK VQPPRLNGAK TGVFSTRSPH121RPNAIGLTLA KLEKVEGGAI YLSGIDMIHG TPVLDIKPYI AEYDSPQNVM EPLADFNLQN181NQHTPNTVSQ SDSKTDSCDQ RQLSGCDEPQ PHHSTKRKPK CPEDRTSEEN YLTHSDTARI241QQAFPMHREI AVDFGLESRR DQSSSVAEEQ IGPYCPEKSF SEKGTDKKLE RVEGAAVLQG301SRAETQPMAP HCPAGRADGA PRSVVPAWVT EAPVATLEVR FTPHAEMDLG QLSSQDVGQA361SFKYFQSAEE AKRAIEAVLS ADPRSVYRRK LCQDRLFYFT VDIAHVTCWF GDGFAEVLRI421KPASEPVHMT GPVGSLVSLG STRTRPLEQK LISEEDLAAN DILDYKDDDD KV
472 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.