Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TRIP10 / CIP4 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G116784-100
Catalog Number: LS-G116784
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Cip4(TRIP10) (NM_004240), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Cdc42-interacting protein 4 | Protein Felic | Salt tolerator | STOT | STP | TR-interacting protein 10 | Salt tolerant protein | TRIP10 | CIP4 | HSTP | TRIP-10
- Origin Species
- Human
- NCBI GenBank
- NM_004240
- SwissProt
- Q15642
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 62.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDWGTELWDQ FEVLERHTQW GLDLLDRYVK FVKERTEVEQ AYAKQLRSLV KKYLPKRPAK61DDPESKFSQQ QSFVQILQEV NDFAGQRELV AENLSVRVCL ELTKYSQEMK QERKMHFQEG121RRAQQQLENG FKQLENSKRK FERDCREAEK AAQTAERLDQ DINATKADVE KAKQQAHLRS181HMAEESKNEY AAQLQRFNRD QAHFYFSQMP QIFDKLQDMD ERRATRLGAG YGLLSEAELE241VVPIIAKCLE GMKVAANAVD PKNDSHVLIE LHKSGFARPG DVEFEDFSQP MNRAPSDSSL301GTPSDGRPEL RGPGRSRTKR WPFGKKNKTV VTEDFSHLPP EQQRKRLQQQ LEERSRELQK361EVDQREALKK MKDVYEKTPQ MGDPASLEPQ IAETLSNIER LKLEVQKYEA WLAEAESRVL421SNRGDSLSRH ARPPDPPASA PPDSSSNSAS QDTKESSEEP PSEESQDTPI YTEFDEDFEE481EPTSPIGHCV AIYHFEGSSE GTISMAEGED LSLMEEDKGD GWTRVRRKEG GEGYVPTSYL541RVTLNTRTRP LEQKLISEED LAANDILDYK DDDDKV
576 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.