Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TRIP / LRRFIP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G116447-100
Catalog Number: LS-G116447
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human LRRFIP1 (NM_004735), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FLAP-1 | FLAP1 | FLIIAP1 | GCF2 | LRR FLII-interacting protein 1 | HUFI-1 | LRRFIP1 | Tcf9 | Tcf-9 | TRIP | NEDD8-conjugating enzyme | TAR RNA-interacting protein | Transcription factor 9 | GC-binding factor 2 | GCF-2
- Origin Species
- Human
- NCBI GenBank
- NM_004735
- SwissProt
- Q32MZ4
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 86.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTSPAAAQSR EIDCLSPEAQ KLAEARLAAK RAARAEAREI RMKELERQQK EEDSERYSRR61SRRNTSASDE DERMSVGSRG SLRVEERPEK DFTEKGSRNM PGLSAATLAS LGGTSSRRGS121GDTSISIDTE ASIREIKDSL AEVEEKYKKA MVSNAQLDNE KTNFMYQVDT LKDMLLELEE181QLAESRRQYE EKNKEFEREK HAHSILQFQF AEVKEALKQR EEMLEKHGII LNSEIATNGE241TSDTLNNVGY QGPTKMTKEE LNALKSTGDG TLGRASEVEV KNEIVANVGK REILHNTEKE301QHTEDTVKDC VDIEVFPAGE NTEDQKSSED TAPFLGTLAG ATYEEQVQSQ ILESSSLPEN361TVQVESNEVM GAPDDRTRTP LEPSNCWSDL DGGNHTENVG EAAVTQVEEQ AGTVASCPLG421HSDDTVYHDD KCMVEVPQEL ETSTGHSLEK EFTNQEAAEP KEVPAHSTEV GRDHNEEEGE481ETGLRDEKPI KTEVPGSPAG TEGNCQEATG PSTVDTQNEP LDMKEPDEEK SDQQGEALDS541SQKKTKNKKK KNKKKKSPVP VETLKDVKKE LTYQNTDLSE IKEEEQVKST DRKSAVEAQN601EVTENPKQKI AAESSENVDC PENPKIKLDG KLDQEGDDVQ TAAEEVLADG DTLDFEDDTV661QSSGPRAGGE ELDEGVAKDN AKIDGATQSS PAEPKSEDAD RCTLPEHESP SQDISDACEA721ESTERCEMSE HPSQTVRKAL DSNSLENDDL SAPGREPGHF NPESREDTRG GNEKGKSKED781CTMSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
784 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.