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TRIM7 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRIM7 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G103156-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RNF90(TRIM7) (NM_203293), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Glycogenin-interacting protein | GNIP | RING finger protein 90 | RNF90 | Tripartite motif protein TRIM7 | Tripartite motif-containing 7 | Tripartite motif containing 7 | TRIM7
Origin Species
Human
NCBI GenBank
NM_033342
SwissProt
Q9C029

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAAVGPRTGP GTGAEALALA AELQGEATCS ICLELFREPV SVECGHSFCR ACIGRCWERP
61GAGSVGAATR APPFPLPCPQ CREPARPSQL RPNRQLAAVA TLLRRFSLPA AAPGEHGSQA
121AAARAAAARC GQHGEPFKLY CQDDGRAICV VCDRAREHRE HAVLPLDEAV QEAKELLESR
181LRVLKKELED CEVFRSTEKK ESKELLKQMA AEQEKVGAEF QALRAFLVEQ EGRLLGRLEE
241LSREVAQKQN ENLAQLGVEI TQLSKLSSQI QETAQKPDLD FLQEFKSTLS RCSNVPGPKP
301TTVSSEMKNK VWNVSLKTFV LKGMLKKFKE DLRGELEKEE KVELTLDPDT ANPRLILSLD
361LKGVRLGERA QDLPNHPCRF DTNTRVLASC GFSSGRHHWE VEVGSKDGWA FGVARESVRR
421KGLTPFTPEE GVWALQLNGG QYWAVTSPER SPLSCGHLSR VRVALDLEVG AVSFYAVEDM
481RHLYTFRVNF QERVFPLFSV CSTGTYLRIW P
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

511 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.