Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TRIM69 / Trif Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G104064-100
Catalog Number: LS-G104064
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RNF36(TRIM69) (NM_182985), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- HSD34 | TRIF | TRIM69 | TRIMLESS | Tripartite motif containing 69 | RING finger protein 36 | Tripartite motif protein 69 | Tripartite motif-containing 69 | RNF36
- Origin Species
- Human
- NCBI GenBank
- NM_182985
- SwissProt
- Q86WT6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 57.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEVSTNPSSN IDPGDYVEMN DSITHLPSKV VIQDITMELH CPLCNDWFRD PLMLSCGHNF61CEACIQDFWR LQAKETFCPE CKMLCQYNNC TFNPVLDKLV EKIKKLPLLK GHPQCPEHGE121NLKLFSKPDG KLICFQCKDA RLSVGQSKEF LQISDAVHFF TEELAIQQGQ LETTLKELQT181LRNMQKEAIA AHKENKLHLQ QHVSMEFLKL HQFLHSKEKD ILTELREEGK ALNEEMELNL241SQLQEQCLLA KDMLVSIQAK TEQQNSFDFL KDITTLLHSL EQGMKVLATR ELISRKLNLG301QYKGPIQYMV WREMQDTLCP GLSPLTLDPK TAHPNLVLSK SQTSVWHGDI KKIMPDDPER361FDSSVAVLGS RGFTSGKWYW EVEVAKKTKW TVGVVRESII RKGSCPLTPE QGFWLLRLRN421QTDLKALDLP SFSLTLTNNL DKVGIYLDYE GGQLSFYNAK TMTHIYTFSN TFMEKLYPYF481CPCLNDGGEN KEPLHILHPQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
500 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.