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TRIM68 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRIM68 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TRIM68 antibodies →
$504.00each
Catalog Number: LS-G101529-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRIM68 (NM_018073), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GC109 | RNF137 | Ro/SSA1 related protein | SS-56 | TRIM68 | Tripartite motif containing 68 | RING finger protein 137 | SS56 | Tripartite motif-containing 68 | SSA protein SS-56
Origin Species
Human
NCBI GenBank
NM_018073
SwissProt
Q6AZZ1

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.1 kda
Protein Type
Lysate
Amino Acid Sequence
1MDPTALVEAI VEEVACPICM TFLREPMSID CGHSFCHSCL SGLWEIPGES QNWGYTCPLC
61RAPVQPRNLR PNWQLANVVE KVRLLRLHPG MGLKGDLCER HGEKLKMFCK EDVLIMCEAC
121SQSPEHEAHS VVPMEDVAWE YKWELHEALE HLKKEQEEAW KLEVGERKRT ATWKIQVETR
181KQSIVWEFEK YQRLLEKKQP PHRQLGAEVA AALASLQREA AETMQKLELN HSELIQQSQV
241LWRMIAELKE RSQRPVRWML QDIQEVLNRS KSWSLQQPEP ISLELKTDCR VLGLREILKT
301YAADVRLDPD TAYSRLIVSE DRKRVHYGDT NQKLPDNPER FYRYNIVLGS QCISSGRHYW
361EVEVGDRSEW GLGVCKQNVD RKEVVYLSPH YGFWVIRLRK GNEYRAGTDE YPILSLPVPP
421RRVGIFVDYE AHDISFYNVT DCGSHIFTFP RYPFPGRLLP YFSPCYSIGT NNTAPLAICS
481LDGED
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

485 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.