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TRIM66 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRIM66 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TRIM66 antibodies →
$725.00each
Catalog Number: LS-G127077-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRIM66 (NM_014818), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C11orf29 | TRIM66 | Tripartite motif-containing 66 | TIF1DELTA | KIAA0298 | TIF1D | Tripartite motif containing 66
Origin Species
Human
NCBI GenBank
AB002296
SwissProt
O15016

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
134.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MARNCSECKE KRAAHILCTY CNRWLCSSCT EEHRHSPVPG GPFFPRAQKG SPGVNGGPGD
61FTLYCPLHTQ EVLKLFCETC DMLTCHSCLV VEHKEHRCRH VEEVLQNQRM LLEGVTTQVA
121HKKSSLQTSA KQIEDRIFEV KHQHRKVENQ IKMAKMVLMN ELNKQANGLI EELEGITNER
181KRKLEQQLQS IMVLNRQFEH VQNFINWAVC SKTSVPFLFS KELIVFQMQR LLETSCNTDP
241GSPWSIRFTW EPNFWTKQLA SLGCITTEGG QMSRADAPAY GGLQGSSPFY QSHQSPVAQQ
301EALSHPSHKF QSPAVCSSSV CCSHCSPVSP SLKGQVPPPS IHPAHSFRQP PEMVPQQLGS
361LQCSALLPRE KELACSPHPP KLLQPWLETQ PPVEQESTSQ RLGQQLTSQP VCIVPPQDVQ
421QGAHAQPTLQ TPSIQVQFGH HQKLKLSHFQ QQPQQQLPPP PPPLPHPPPP LPPPPQQPHP
481PLPPSQHLAS SQHESPPGPA CSQNMDIMHH KFELEEMQKD LELLLQAQQP SLQLSQTKSP
541QHLQQTIVGQ INYIVRQPAP VQSQSQEETL QATDEPPASQ GSKPALPLDK NTAAALPQAS
601GEETPLSVPP VDSTIQHSSP NVVRKHSTSL SIMGFSNTLE MELSSTRLER PLEPQIQSVS
661NLTAGAPQAV PSLLSAPPKM VSSLTSVQNQ AMPSLTTSHL QTVPSLVHST FQSMPNLISD
721SPQAMASLAS DHPQAGPSLM SGHTQAVPSL ATCPLQSIPP VSDMQPETGS SSSSGRTSGS
781LCPRDGADPS LENALCKVKL EEPINLSVKK PPLAPVVSTS TALQQYQNPK ECENFEQGAL
841ELDAKENQSI RAFNSEHKIP YVRLERLKIC AASSGEMPVF KLKPQKNDQD GSFLLIIECG
901TESSSMSIKV SQDRLSEATQ APGLEGRKVT VTSLAGQRPP EVEGTSPEEH RLIPRTPGAK
961KGPPAPIENE DFCAVCLNGG ELLCCDRCPK VFHLSCHVPA LLSFPGGEWV CTLCRSLTQP
1021EMEYDCENAC YNQPGMRASP GLSMYDQKKC EKLVLSLCCN NLSLPFHEPV SPLARHYYQI
1081IKRPMDLSII RRKLQKKDPA HYTTPEEVVS DVRLMFWNCA KFNYPDSEVA EAGRCLEVFF
1141EGWLKEIYPE KRFAQPRQED SDSEEVSSES GCSTPQGFPW PPYMQEGIQP KRRRRHMENE
1201RAKRMSFRLA NSISQV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1216 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.