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TRIM6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRIM6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TRIM6 antibodies →
$725.00each
Catalog Number: LS-G102186-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RNF89(TRIM6) (NM_058166), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
RING finger protein 89 | RNF89 | TRIM6 | Tripartite motif containing 6 | Tripartite motif-containing 6
Origin Species
Human
NCBI GenBank
AF220030
SwissProt
Q9C030

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
56.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTSPVLVDIR EEVTCPICLE LLTEPLSIDC GHSFCQACIT PNGRESVIGQ EGERSCPVCQ
61TSYQPGNLRP NRHLANIVRR LREVVLGPGK QLKAVLCADH GEKLQLFCQE DGKVICWLCE
121RSQEHRGHHT FLVEEVAQEY QKFQESLKKL KNEEQEAEKL TAFIREKKTS WKNQMEPERC
181RIQTEFNQLR NILDRVEQRE LKKLEQEEKK GLRIIEEAEN DLVHQTQSLR ELISDLERRC
241QGSTMELLQD VSDVTERSEF WTLRKPEALP TKLRSMFRAP DLKRMLRVCR ELTDVQSYWV
301DVTLNPHTAN LNLVLAKNRR QVRFVGAKVS GPSCLEKHYD CSVLGSQHFS SGKHYWEVDV
361AKKTAWILGV CSNSLGPTFS FNHFAQNHSA YSRYQPQSGY WVIGLQHNHE YRAYEDSSPS
421LLLSMTVPPR RVGVFLDYEA GTVSFYNVTN HGFPIYTFSK YYFPTTLCPY FNPCNCVIPM
481TLRRPSS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

487 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.