Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TRIM55 / MURF2 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G103974-100
Catalog Number: LS-G103974
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MURF2(TRIM55) (NM_184086), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- MURF-2 | MuRF2 | RING finger protein 29 | RNF29 | TRIM55 | Tripartite motif containing 55 | Muscle specific ring finger 2 | Tripartite motif-containing 55
- Origin Species
- Human
- SwissProt
- Q9BYV6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 50.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MSASLNYKSF SKEQQTMDNL EKQLICPICL EMFTKPVVIL PCQHNLCRKC ASDIFQASNP61YLPTRGGTTM ASGGRFRCPS CRHEVVLDRH GVYGLQRNLL VENIIDIYKQ ESTRPEKKSD121QPMCEEHEEE RINIYCLNCE VPTCSLCKVF GAHKDCQVAP LTHVFQRQKS ELSDGIAILV181GSNDRVQGVI SQLEDTCKTI EECCRKQKQE LCEKFDYLYG ILEERKNEMT QVITRTQEEK241LEHVRALIKK YSDHLENVSK LVESGIQFMD EPEMAVFLQN AKTLLKKISE ASKAFQMEKI301EHGYENMNHF TVNLNREEKI IREIDFYRED EDEEEEEGGE GEKEGEGEVG GEAVEVEEVE361NVQTEFPGED ENPEKASELS QVELQAAPGA LPVSSPEPPP ALPPAADAPV TQIGFEAPPL421QGQAAAPASG SGADSEPARH IFSFSWLNSL NETRTRPLEQKLISEEDLAANDILDYKDDDDKV
452 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.