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TRIM49 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRIM49 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TRIM49 antibodies →
$725.00each
Catalog Number: LS-G111210-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRIM49 (NM_020358), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TRIM49L2 | TRIM49 | Tripartite motif containing 49 | Tripartite motif-containing 49 | RING finger protein 18 | RNF18 | TRIM49A
Origin Species
Human
NCBI GenBank
NM_020358
SwissProt
Q9NS80

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
52.7 kda
Protein Type
Lysate
Amino Acid Sequence
1MNSGILQVFQ GELICPLCMN YFIDPVTIDC GHSFCRPCFY LNWQDIPFLV QCSECTKSTE
61QINLKTNIHL KKMASLARKV SLWLFLSSEE QMCGTHRETK KIFCEVDRSL LCLLCSSSQE
121HRYHRHRPIE WAAEEHREKL LQKMQSLWEK ACENHRNLNV ETTRTRCWKD YVNLRLEAIR
181AEYQKMPAFH HEEEKHNLEM LKKKGKEIFH RLHLSKAKMA HRMEILRGMY EELNEMCHKP
241DVELLQAFGD ILHRSESVLL HMPQPLNPEL SAGPITGLRD RLNQFRVHIT LHHEEANSDI
301FLYEILRSMC IGCDHQDVPY FTATPRSFLA WGVQTFTSGK YYWEVHVGDS WNWAFGVCNM
361YRKEKNQNEK IDGKEGLFLL GCIKNDIQCS LFTTSPLMLQ YIPKPTSRVG LFLDCEAKTV
421SFVDVNQSSL IYTIPNCSFS PPLRPIFCCI HF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

452 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.