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TRIM47 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRIM47 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G108216-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRIM47 (NM_033452), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
GOA | RING finger protein 100 | RNF100 | TRIM47 | Tripartite motif-containing 47 | Tripartite motif containing 47
Origin Species
Human
SwissProt
Q96LD4

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
69.4 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDGSGPFSCP ICLEPLREPV TLPCGHNFCL ACLGALWPHR GASGAGGPGG AARCPLCQEP
61FPDGLQLRKN HTLSELLQLR QGSGPGSGPG PAPALAPEPS APSALPSVPE PSAPCAPEPW
121PAGEEPVRCD ACPEGAALPA ALSCLSCLAS FCPAHLGPHE RSPALRGHRL VPPLRRLEES
181LCPRHLRPLE RYCRAERVCL CEACAAQEHR GHELVPLEQE RALQEAEQSK VLSAVEDRMD
241ELGAGIAQSR RTVALIKSAA VAERERVSRL FADAAAALQG FQTQVLGFIE EGEAAMLGRS
301QGDLRRQEEQ RSRLSRARQN LSQVPEADSV SFLQELLALR LALEDGCGPG PGPPRELSFT
361KSSQAVRAVR DMLAVACVNQ WEQLRGPGGN EDGPQKLDSE ADAEPQDLES TNLLESEAPR
421DYFLKFAYIV DLDSDTADKF LQLFGTKGVK RVLCPINYPL SPTRFTHCEQ VLGEGALDRG
481TYYWEVEIIE GWVSMGVMAE DFSPQEPYDR GRLGRNAHSC CLQWNGRSFS VWFHGLEAPL
541PHPFSPTVGV CLEYADRALA FYAVRDGKMS LLRRLKASRP RRGGIPASPI DPFQSRLDSH
601FAGLFTHRLK PAFFLESVDA HLQIGPLKKS CISVLKRR
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

638 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.