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TRIM42 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRIM42 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TRIM42 antibodies →
$725.00each
Catalog Number: LS-G106147-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRIM42 (NM_152616), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PPP1R40 | Tripartite motif containing 42 | Tripartite motif-containing 42 | TRIM42
Origin Species
Human
NCBI GenBank
NM_152616
SwissProt
Q8IWZ5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.6 kda
Protein Type
Lysate
Amino Acid Sequence
1METAMCVCCP CCTWQRCCPQ LCSCLCCKFI FTSERNCTCF PCPYKDERNC QFCHCTCSES
61PNCHWCCCSW ANDPNCKCCC TASSNLNCYY YESRCCRNTI ITFHKGRLRS IHTSSKTALR
121TGSSDTQVDE VKSIPANSHL VNHLNCPMCS RLRLHSFMLP CNHSLCEKCL RQLQKHAEVT
181ENFFILICPV CDRSHCMPYS NKMQLPENYL HGRLTKRYMQ EHGYLKWRFD RSSGPILCQV
241CRNRRIAYKR CITCRLNLCN DCLKAFHSDV AMQDHVFVDT SAEEQDEKIC IHHPSSRIIE
301YCRNDNKLLC TFCKFSFHNG HDTISLIDAC SERAASLFSA IAKFKAVRYE IDNDLMEFNI
361LKNSFKADKE AKRKEIRNGF LKLRSILQEK EKIIMEQIEN LEVSRQKEIE KYVYVTTMKV
421NEMDGLIAYS KEALKETGQV AFLQSAKILV DQIEDGIQTT YRPDPQLRLH SINYMPLDFV
481ELSSAIHELF PTGPKKVRSS GDSLPSPYPV HSETMIARKV TFSTHSLGNQ HIYQRSSSML
541SFSNTDKKAK VGLEACGRAQ SATPAKPTDG LYTYWSAGED SQSVQNSSSF HNWYSFNDGS
601VKTPGPIVIY QTLVYPRAAK VYWTCPAEDV DSFEMEFYEV ITSPPNNVQM ELCGQIRDIM
661QQNLELHNLT PNTEYVFKVR AINDNGPGQW SDICKVVTPD GHGKNRAKWG LLKNIQSALQ
721KHF
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

723 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.