Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TRIM4 / RNF87 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G108439-100
Catalog Number: LS-G108439
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RNF87(TRIM4) (NM_033091), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- RING finger protein 87 | Tripartite motif protein 4 | TRIM4 | Tripartite motif-containing 4 | RNF87 | Tripartite motif containing 4
- Origin Species
- Human
- NCBI GenBank
- AF220023
- SwissProt
- Q9C037
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 54 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEAEDIQEEL TCPICLDYFQ DPVSIECGHN FCRGCLHRNW APGGGPFPCP ECRHPSAPAA61LRPNWALARL TEKTQRRRLG PVPPGLCGRH WEPLRLFCED DQRPVCLVCR ESQEHQTHAM121APIDEAFESY REKLLKSQRN LVAKMKKVMH LQDVEVKNAT QWKDKIKSQR MRISTEFSKL181HNFLVEEEDL FLQRLNKEEE ETKKKLNENT LKLNQTIASL KKLILEVGEK SQAPTLELLQ241NPKEVLTRSE IQDVNYSLEA VKVKTVCQIP LMKEMLKRFQ VAVNLAEDTA HPKLVFSQEG301RYVKNTASAS SWPVFSSAWN YFAGWRNPQK TAFVERFQHL PCVLGKNVFT SGKHYWEVES361RDSLEVAVGV CREDVMGITD RSKMSPDVGI WAIYWSAAGY WPLIGFPGTP TQQEPALHRV421GVYLDRGTGN VSFYSAVDGV HLHTFSCSSV SRLRPFFWLS PLASLVIPPV TDRKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
474 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.