Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TRIM39 / RNF23 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G131000-100
Catalog Number: LS-G131000
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRIM39 (NM_001199119), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- TRIM39 | Tripartite motif containing 39 | Testis-abundant finger protein | TRIM39B | RING finger protein 23 | RNF23 | Tripartite motif-containing 39 | TFP
- Origin Species
- Human
- NCBI GenBank
- NM_021253
- SwissProt
- Q9HCM9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAETSLLEAG ASAASTAAAL ENLQVEASCS VCLEYLKEPV IIECGHNFCK ACITRWWEDL61ERDFPCPVCR KTSRYRSLRP NRQLGSMVEI AKQLQAVKRK IRDESLCPQH HEALSLFCYE121DQEAVCLICA ISHTHRAHTV VPLDDATQEY KEKLQKCLEP LEQKLQEITR CKSSEEKKPG181ELKRLVESRR QQILREFEEL HRRLDEEQQV LLSRLEEEEQ DILQRLRENA AHLGDKRRDL241AHLAAEVEGK CLQSGFEMLK DVKSTLEKCE KVKTMEVTSV SIELEKNFSN FPRQYFALRK301ILKQLIADVT LDPETAHPNL VLSEDRKSVK FVETRLRDLP DTPRRFTFYP CVLATEGFTS361GRHYWEVEAA HCVLAQDPEN QALARFYCYT ERTIAKRLVL RRDPSVKRTL CRGCSSLLVP421GLTCTQRQRR CRGQRWTVQT CLTCQRSQRF LNDPGHLLWG DRPEAQLGSQ ADSKPLQPLP481NTAHSISDRL PEEKMQTQGS SNQTRTRPLEQKLISEEDLAANDILDYKDDDDKV
503 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.