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TRIM36 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRIM36 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TRIM36 antibodies →
$725.00each
Catalog Number: LS-G111575-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RNF98(TRIM36) (NM_018700), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
HAPRIN | Tripartite motif containing 36 | RING finger protein 98 | RNF98 | TRIM36 | Zinc-binding protein Rbcc728 | RBCC728 | Tripartite motif protein 36 | Tripartite motif-containing 36
Origin Species
Human
NCBI GenBank
NM_018700
SwissProt
Q9NQ86

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
82.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSESGEMSEF GYIMELIAKG KVTIKNIERE LICPACKELF THPLILPCQH SICHKCVKEL
61LLTLDDSFND VGSDNSNQSS PRLRLPSPSM DKIDRINRPG WKRNSLTPRT TVFPCPGCEH
121DVDLGERGIN GLFRNFTLET IVERYRQAAR AATAIMCDLC KPPPQESTKS CMDCSASYCN
181ECFKIHHPWG TIKAQHEYVG PTTNFRPKIL MCPEHETERI NMYCELCRRP VCHLCKLGGN
241HANHRVTTMS SAYKTLKEKL SKDIDYLIGK ESQVKSQISE LNLLMKETEC NGERAKEEAI
301THFEKLFEVL EERKSSVLKA IDSSKKLRLD KFQTQMEEYQ GLLENNGLVG YAQEVLKETD
361QSCFVQTAKQ LHLRIQKATE SLKSFRPAAQ TSFEDYVVNT SKQTELLGEL SFFSSGIDVP
421EINEEQSRVY NNALINWHHP EKDKADSYVL EYRKINRDDE MSWNEIEVCG TSKIIQDLEN
481SSTYAFRVRA YKGSICSPCS RELILHTPPA PVFSFLFDEK CGYNNEHLLL NLKRDRVESR
541AGFNLLLAAE RIQVGYYTSL DYIIGDTGIT KGKHFWAFRV EPYSYLVKVG VASSDKLQEW
601LRSPRDAVSP RYEQDSGHDS GSEDACFDSS QPFTLVTIGM QKFFIPKSPT SSNEPENRVL
661PMPTSIGIFL DCDKGKVNFY DMDQMKCLYE RQVDCSHTLY PAFALMGSGG IQLEEPITAK
721YLEYQEDM
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

728 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.