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TRIM26 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRIM26 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TRIM26 antibodies →
$504.00each
Catalog Number: LS-G117331-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRIM26 (NM_003449), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Acid finger protein | TRIM26 | ZNF173 | RING finger protein 95 | RNF95 | Tripartite motif containing 26 | Zinc finger protein 173 | Tripartite motif-containing 26
Origin Species
Human
NCBI GenBank
NM_003449
SwissProt
Q12899

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
62 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATSAPLRSL EEEVTCSICL DYLRDPVTID CGHVFCRSCT TDVRPISGSR PVCPLCKKPF
61KKENIRPVWQ LASLVENIER LKVDKGRQPG EVTREQQDAK LCERHREKLH YYCEDDGKLL
121CVMCRESREH RPHTAVLMEK AAQPHREKIL NHLSTLRRDR DKIQGFQAKG EADILAALKK
181LQDQRQYIVA EFEQGHQFLR EREEHLLEQL AKLEQELTEG REKFKSRGVG ELARLALVIS
241ELEGKAQQPA AELMQDTRDF LNRYPRKKFW VGKPIARVVK KKTGEFSDKL LSLQRGLREF
301QGKLLRDLEY KTVSVTLDPQ SASGYLQLSE DWKCVTYTSL YKSAYLHPQQ FDCEPGVLGS
361KGFTWGKVYW EVEVEREGWS EDEEEGDEEE EGEEEEEEEE AGYGDGYDDW ETDEDEESLG
421DEEEEEEEEE EEVLESCMVG VARDSVKRKG DLSLRPEDGV WALRLSSSGI WANTSPEAEL
481FPALRPRRVG IALDYEGGTV TFTNAESQEL IYTFTATFTR RLVPFLWLKW PGTRLLLRP
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

539 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.