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TRIM13 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRIM13 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TRIM13 antibodies →
$504.00each
Catalog Number: LS-G121986-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human RFP2(TRIM13) (NM_001007278), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DLEU5 | CLL-associated RING finger | LEU5 | Leukemia-associated protein 5 | RING finger protein 77 | Tripartite motif protein 13 | Putative tumor suppressor RFP2 | RFP2 | TRIM13 | CAR | Ret finger protein 2 | RNF77 | Tripartite motif containing 13 | Tripartite motif-containing 13
Origin Species
Human
NCBI GenBank
NM_005798
SwissProt
O60858

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
47.2 kda
Protein Type
Lysate
Amino Acid Sequence
1MDVMELLEED LTCPICCSLF DDPRVLPCSH NFCKKCLEGI LEGSVRNSLW RPAPFKCPTC
61RKETSATGIN SLQVNYSLKG IVEKYNKIKI SPKMPVCKGH LGQPLNIFCL TDMQLICGIC
121ATRGEHTKHV FCSIEDAYAQ ERDAFESLFQ SFETWRRGDA LSRLDTLETS KRKSLQLLTK
181DSDKVKEFFE KLQHTLDQKK NEILSDFETM KLAVMQAYDP EINKLNTILQ EQRMAFNIAE
241AFKDVSEPIV FLQQMQEFRE KIKVIKETPL PPSNLPASPL MKNFDTSQWE DIKLVDVDKL
301SLPQDTGTFI SKIPWSFYKL FLLILLLGLV IVFGPTMFLE WSLFDDLATW KGCLSNFSSY
361LTKTADFIEQ SVFYWEQVTD GFFIFNERFK NFTLVVLNNV AEFVCKYKLL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

410 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.