Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TRIM1 / MID2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G108175-100
Catalog Number: LS-G108175
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human MID2 (NM_052817), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FXY2 | Midin-2 | Midline-2 | MID2 | Midin 2 | Midline 2 | Midline defect 2 | RNF60 | RING finger protein 60 | TRIM1 | Tripartite motif protein 1
- Origin Species
- Human
- NCBI GenBank
- NM_012216
- SwissProt
- Q9UJV3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 77.7 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1METLESELTC PICLELFEDP LLLPCAHSLC FSCAHRILVS SCSSGESIEP ITAFQCPTCR61YVISLNHRGL DGLKRNVTLQ NIIDRFQKAS VSGPNSPSES RRERTYRPTT AMSSERIACQ121FCEQDPPRDA VKTCITCEVS YCDRCLRATH PNKKPFTSHR LVEPVPDTHL RGITCLDHEN181EKVNMYCVSD DQLICALCKL VGRHRDHQVA SLNDRFEKLK QTLEMNLTNL VKRNSELENQ241MAKLIQICQQ VEVNTAMHEA KLMEECDELV EIIQQRKQMI AVKIKETKVM KLRKLAQQVA301NCRQCLERST VLINQAEHIL KENDQARFLQ SAKNIAERVA MATASSQVLI PDINFNDAFE361NFALDFSREK KLLEGLDYLT APNPPSIREE LCTASHDTIT VHWISDDEFS ISSYELQYTI421FTGQANFISL YNSVDSWMIV PNIKQNHYTV HGLQSGTRYI FIVKAINQAG SRNSEPTRLK481TNSQPFKLDP KMTHKKLKIS NDGLQMEKDE SSLKKSHTPE RFSGTGCYGA AGNIFIDSGC541HYWEVVMGSS TWYAIGIAYK SAPKNEWIGK NASSWVFSRC NSNFVVRHNN KEMLVDVPPH601LKRLGVLLDY DNNMLSFYDP ANSLHLHTFD VTFILPVCPT FTIWNKSLMI LSGLPAPDFI661DYPERQECNC RPQESPYVSG MKTCHTRTRPLEQKLISEEDLAANDILDYKDDDDKV
685 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.