Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TRAPPC13 / C5orf44 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G109663-100
Catalog Number: LS-G109663
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C5ORF44(TRAPPC13) (NM_024941), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- FLJ13611 | UPF0533 protein C5orf44 | C5orf44
- Origin Species
- Human
- NCBI GenBank
- NM_024941
- SwissProt
- A5PLN9
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEVNPPKQEH LLALKVMRLT KPTLFTNIPV TCEEKDLPGD LFNQLMRDDP STVNGAEVLM61LGEMLTLPQN FGNIFLGETF SSYISVHNDS NQVVKDILVK ADLQTSSQRL NLSASNAAVA121ELKPDCCIDD VIHHEVKEIG THILVCAVSY TTQAGEKMYF RKFFKFQVLK PLDVKTKFYN181AESDLSSVTD EVFLEAQIQN MTTSPMFMEK VSLEPSIMYN VTELNSVSQA GECVSTFGSR241AYLQPMDTRQ YLYCLKPKNE FAEKAGIIKG VTVIGKLDIV WKTNLGERGR LQTSQLQRMA301PGYGDVRLSL EAIPDTVNLE EPFHITCKIT NCSERTMDLV LEMCNTNSIH WCGISGRQLG361KLHPSSSLCL ALTLLSSVQG LQSISGLRLT DTFLKRTYEY DDIAQVCVVS SAIKVESTRTRPLEQKLISEEDLAANDILDYKDDDDKV
417 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.