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TRAPPC11 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRAPPC11 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G110560-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRAPPC11 (NM_021942), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
C4orf41 | Foie gras homolog | FLJ12716 | Gry | Gryzun homolog | Foigr | TRAPPC11
Origin Species
Human
NCBI GenBank
NM_021942
SwissProt
Q7Z392

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
128.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSPTQWDFPV ELCCRPMAFV TLTGLDVVYN AVHRAVWDAF CANRRADRVP ISFKVLPGDH
61EYPKCRPKRT SYEWYIPKGI LKTGWMNKHL NLVPALVVVF YELDWDEPQW KEKQSECATR
121VEIVRQSLQG RNTKVAVVLI QKKTPLPPGE DVIASERAAA LCNACELSGK SLFVLPHTDH
181LVGYIIRLEN AFYEHAQTYY YTEIRRVKSH KEFLNKTTHQ LLFVRHQFKI AFFSELKQDT
241QNALKNYRTA YNLVHELRAH ETNILEIKTM AGFINYKICR LCFQHNTPLD AIAQFRKHID
301LCKKKIGSAE LSFEHDAWMS KQFQAFGDLF DEAIKLGLTA IQTQNPGFYY QQAAYYAQER
361KQLAKTLCNH EASVMYPNPD PLETQTGVLD FYGQRSWRQG ILSFDLSDPE KEKVGILAIQ
421LKERNVVHSE IIITLLSNAV AQFKKYKCPR MKSHLMVQMG EEYYYAKDYT KALKLLDYVM
481CDYRSEGWWT LLTSVLTTAL KCSYLMAQLK DYITYSLELL GRASTLKDDQ KSRIEKNLIN
541VLMNESPDPE PDCDILAVKT AQKLWADRIS LAGSNIFTIG VQDFVPFVQC KAKFHAPSFH
601VDVPVQFDIY LKADCPHPIR FSKLCVSFNN QEYNQFCVIE EASKANEVLE NLTQGKMCLV
661PGKTRKLLFK FVAKTEDVGK KIEITSVDLA LGNETGRCVV LNWQGGGGDA ASSQEALQAA
721RSFKRRPKLP DNEVHWDSII IQASTMIISR VPNISVHLLH EPPALTNEMY CLVVTVQSHE
781KTQIRDVKLT AGLKPGQDAN LTQKTHVTLH GTELCDESYP ALLTDIPVGD LHPGEQLEKM
841LYVRCGTVGS RMFLVYVSYL INTTVEEKEI VCKCHKDETV TIETVFPFDV AVKFVSTKFE
901HLERVYADIP FLLMTDLLSA SPWALTIVSS ELQLAPSMTT VDQLESQVDN VILQTGESAS
961ECFCLQCPSL GNIEGGVATG HYIISWKRTS AMENIPIITT VITLPHVIVE NIPLHVNADL
1021PSFGRVRESL PVKYHLQNKT DLVQDVEISV EPSDAFMFSG LKQIRLRILP GTEQEMLYNF
1081YPLMAGYQQL PSLNINLLRF PNFTNQLLRR FIPTSIFVKP QGRLMDDTSI AAA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1133 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.