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TRAK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRAK1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G113561-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRAK1 (NM_014965), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
KIAA1042 | Milton homolog 1 | OIP106 | TRAK1 | MILT1
Origin Species
Human
SwissProt
Q9UPV9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
77.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSLRDKGGEE ECFEYDCQDE ERKPTHRQHD TQDLLEEVLC AERVGQMTKT YNDIDAVTRL
61LEEKERDLEL AARIGQSLLK KNKTLTERNE LLEEQVEHIR EEVSQLRHEL SMKDELLQFY
121TSAAEESEPE SVCSTPLKRN ESSSSVQNYF HLDSLQKKLK DLEEENVVLR SEASQLKTET
181ITYEEKEQQL VNDCVKELRD ANVQIASISE ELAKKTEDAA RQQEEITHLL SQIVDLQKKA
241KACAVENEEL VQHLGAAKDA QRQLTAELRE LEDKYAECME MLHEAQEELK NLRNKTMPNT
301TSRRYHSLGL FPMDSLAAEI EGTMRKELQL EEAESPDITH QKRVFETVRN INQVVKQRSL
361TPSPMNIPGS NQSSAMNSLL SSCVSTPRSS FYGSDIGNVV LDNKTNSIIL ETEAADLGND
421ERSKKPGTPG TPGSHDLETA LRRLSLRREN YLSERRFFEE EQERKLQELA EKGELRSGSL
481TPTESIMSLG THSRFSEFTG FSGMSFSSRS YLPEKLQIVK PLEGSATLHH WQQLAQPHLG
541GILDPRPGVV TKGFRTLDVD LDEVYCLNDF EEDDTGDHIS LPRLATSTPV QHPETSGERS
601QARVTVSGSR SYPSRPQASP EEMQEPPAAT EEEEEEEEEE EEGSGEGTTI SPVNLAPFPE
661AEFWAILTSV PGTIRSGSLS VASARLCG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

688 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.