Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TRAIP / TRIP Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G115662-100
Catalog Number: LS-G115662
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRIP(TRAIP) (NM_005879), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- RING finger protein 206 | RNF206 | TRAF interacting protein | TRIP | TRAF-interacting protein | TRAIP
- Origin Species
- Human
- NCBI GenBank
- NM_005879
- SwissProt
- Q9BWF2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 53.1 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MPIRALCTIC SDFFDHSRDV AAIHCGHTFH LQCLIQWFET APSRTCPQCR IQVGKRTIIN61KLFFDLAQEE ENVLDAEFLK NELDNVRAQL SQKDKEKRDS QVIIDTLRDT LEERNATVVS121LQQALGKAEM LCSTLKKQMK YLEQQQDETK QAQEEARRLR SKMKTMEQIE LLLQSQRPEV181EEMIRDMGVG QSAVEQLAVY CVSLKKEYEN LKEARKASGE VADKLRKDLF SSRSKLQTVY241SELDQAKLEL KSAQKDLQSA DKEIMSLKKK LTMLQETLNL PPVASETVDR LVLESPAPVE301VNLKLRRPSF RDDIDLNATF DVDTPPARPS SSQHGYYEKL CLEKSHSPIQ DVPKKICKGP361RKESQLSLGG QSCAGEPDEE LVGAFPIFVR NAILGQKQPK RPRSESSCSK DVVRTGFDGL421GGRTKFIQPT DTVMIRPLPV KPKTKVKQRV RVKTVPSLFQ AKLDTFLWSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
469 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.