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TRAF5 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TRAF5 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G120972-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TRAF5 (NM_001033910), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
TRAF5 | RNF84 | MGC:39780 | RING finger protein 84
Origin Species
Human
NCBI GenBank
NM_004619
SwissProt
O00463

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAYSEEHKGM PCGFIRQNSG NSISLDFEPS IEYQFVERLE ERYKCAFCHS VLHNPHQTGC
61GHRFCQHCIL SLRELNTVPI CPVDKEVIKS QEVFKDNCCK REVLNLYVYC SNAPGCNAKV
121ILGRYQDHLQ QCLFQPVQCS NEKCREPVLR KDLKEHLSAS CQFRKEKCLY CKKDVVVINL
181QNHEENLCPE YPVFCPNNCA KIILKTEVDE HLAVCPEAEQ DCPFKHYGCA VTDKRRNLQQ
241HEHSALREHM RLVLEKNVQL EEQISDLHKS LEQKESKIQQ LAETIKKLEK EFKQFAQLFG
301KNGSFLPNIQ VFASHIDKSA WLEAQVHQLL QMVNQQQNKF DLRPLMEAVD TVKQKITLLE
361NNDQRLAVLE EETNKHDTHI NIHKAQLSKN EERFKLLEGT CYNGKLIWKV TDYKMKKREA
421VDGHTVSIFS QSFYTSRCGY RLCARAYLNG DGSGRGSHLS LYFVVMRGEF DSLLQWPFRQ
481RVTLMLLDQS GKKNIMETFK PDPNSSSFKR PDGEMNIASG CPRFVAHSVL ENAKNAYIKD
541DTLFLKVAVD LTDLEDL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

557 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.