Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TRAF3IP2 / ACT1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G106518-100
Catalog Number: LS-G106518
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human CIKS(TRAF3IP2) (NM_147686), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C6orf2 | C6orf4 | C6orf5 | CIKS | DKFZP586G0522 | Connection to IKK and SAPK/JNK | ACT1 | Adapter protein CIKS | NFkB-activating protein ACT1 | PSORS13 | TRAF3-interacting protein 2 | TRAF3IP2 | C6orf6 | TRAF3 interacting protein 2
- Origin Species
- Human
- NCBI GenBank
- NM_147200
- SwissProt
- O43734
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 63.4 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MNRSIPVEVD ESEPYPSQLL KPIPEYSPEE ESEPPAPNIR NMAPNSLSAP TMLHNSSGDF61SQAHSTLKLA NHQRPVSRQV TCLRTQVLED SEDSFCRRHP GLGKAFPSGC SAVSEPASES121VVGALPAEHQ FSFMEKRNQW LVSQLSAASP DTGHDSDKSD QSLPNASADS LGGSQEMVQR181PQPHRNRAGL DLPTIDTGYD SQPQDVLGIR QLERPLPLTS VCYPQDLPRP LRSREFPQFE241PQRYPACAQM LPPNLSPHAP WNYHYHCPGS PDHQVPYGHD YPRAAYQQVI QPALPGQPLP301GASVRGLHPV QKVILNYPSP WDQEERPAQR DCSFPGLPRH QDQPHHQPPN RAGAPGESLE361CPAELRPQVP QPPSPAAVPR PPSNPPARGT LKTSNLPEEL RKVFITYSMD TAMEVVKFVN421FLLVNGFQTA IDIFEDRIRG IDIIKWMERY LRDKTVMIIV AISPKYKQDV EGAESQLDED481EHGLHTKYIH RMMQIEFIKQ GSMNFRFIPV LFPNAKKEHV PTWLQNTHVY SWPKNKKNIL541LRLLREEEYV APPRGPLPTL QVVPLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
565 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.