Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TRABD2A / C2orf89 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G119713-100
Catalog Number: LS-G119713
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C2orf89(TRABD2A) (NM_001080824), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C2orf89 | TRABD2A | TIKI1 | TraB domain containing 2A | UPF0632 protein C2orf89
- Origin Species
- Human
- NCBI GenBank
- NM_001080824
- SwissProt
- Q86V40
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 52.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSPWSWFLLQ TLCLLPTGAA SRRGAPGTAN CELKPQQSEL NSFLWTIKRD PPSYFFGTIH61VPYTRVWDFI PDNSKEAFLQ SSIVYFELDL TDPYTISALT SCQMLPQGEN LQDVLPRDIY121CRLKRHLEYV KLMMPLWMTP DQRGKGLYAD YLFNAIAGNW ERKRPVWVML MVNSLTEVDI181KSRGVPVLDL FLAQEAERLR KQTGAVEKVE EQCHPLNGLN FSQVPNFINA TLPPQERITA241QEIDSYLRRE LIYKRNERIG KRVKALLEEF PDKGFFFAFG AGHFMGNNTV LDVLRREGYE301VEHAPAGRPI HKGKSKKTST RPTLSTIFAP KVPTLEVPAP EAVSSGHSTL PPLVSRPGSA361DTPSEAEQRF RKKRRRSQRR PRLRQFSDLW VRLEESDIVP QLQVPVLDRH ISTELRLPRR421GHSHHSQMVA SSACLSLWTP VFWVLVLAFQ TETPLLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
456 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.