Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TPSG1 / Tryptase Gamma 1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101102-100
Catalog Number: LS-G101102
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TPSG1 (NM_012467), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Mast cell protease II | Mast cell tryptase | Pituitary tryptase | Skin tryptase | Lung tryptase | TPSG1 | TrpA | Tryptase gamma I | Transmembrane tryptase | Tryptase gamma | Tryptase gamma 1 | PRSS31 | TMT | Gamma I | Gamma II | Serine protease 31 | Tryptase gamma II
- Origin Species
- Human
- NCBI GenBank
- NM_012467
- SwissProt
- Q9NRR2
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 33.76 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MALGACGLLL LLAVPGVSLR TLQPGCGRPQ VSDAGGRIVG GHAAPAGAWP WQASLRLRRM61HVCGGSLLSP QWVLTAAHCF SGSLNSSDYQ VHLGELEITL SPHFSTVRQI ILHSSPSGQP121GTSGDIALVE LSVPVTLSSR ILPVCLPEAS DDFCPGIRCS VTGWGYTREG EPLPPPYSLR181EVKVSVVDTE TCRRDYPGPG GSILQPDMLC ARGPGDACQD DSGGPLVCQV NGAWVQAGIV241SWGEGCGRPN RPGVYTRVPA YVNWIRRHIT ASGGSESGYP RLPLLAGFFL PGLFLLLVSC301VLLAKCLLHP SADGTPFPAP DTRTRPLEQKLISEEDLAANDILDYKDDDDKV
321 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.