Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TOX4 / C14orf92 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G113699-100
Catalog Number: LS-G113699
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TOX4 (NM_014828), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Migration-inducing protein 7 | TOX4 | C14orf92 | KIAA0737 | MIG7
- Origin Species
- Human
- NCBI GenBank
- AB018280
- SwissProt
- O94842
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 66 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEFPGGNDNY LTITGPSHPF LSGAETFHTP SLGDEEFEIP PISLDSDPSL AVSDVVGHFD61DLADPSSSQD GSFSAQYGVQ TLDMPVGMTH GLMEQGGGLL SGGLTMDLDH SIGTQYSANP121PVTIDVPMTD MTSGLMGHSQ LTTIDQSELS SQLGLSLGGG TILPPAQSPE DRLSTTPSPT181SSLHEDGVED FRRQLPSQKT VVVEAGKKQK APKKRKKKDP NEPQKPVSAY ALFFRDTQAA241IKGQNPNATF GEVSKIVASM WDSLGEEQKQ VYKRKTEAAK KEYLKALAAY KDNQECQATV301ETVELDPAPP SQTPSPPPMA TVDPASPAPA SIEPPALSPS IVVNSTLSSY VANQASSGAG361GQPNITKLII TKQMLPSSIT MSQGGMVTVI PATVVTSRGL QLGQTSTATI QPSQQAQIVT421RSVLQAAAAA AAAASMQLPP PRLQPPPLQQ MPQPPTQQQV TILQQPPPLQ AMQQPPPQKV481RINLQQQPPP LQIKSVPLPT LKMQTTLVPP TVESSPERPM NNSPEAHTVE APSPETICEM541ITDVVPEVES PSQMDVELVS GSPVALSPQP RCVRSGCENP PIVSKDWDNE YCSNECVVKH601CRDVFLAWVA SRNSNTVVFV KTRTRPLEQKLISEEDLAANDILDYKDDDDKV
621 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.