Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TOR4A / C9orf167 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G112266-100
Catalog Number: LS-G112266
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C9orf167(TOR4A) (NM_017723), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- C9orf167 | RP13-122B23.4 | Torsin family 4 | member A | Torsin family protein C9orf167 | Torsin family 4 member A | Torsin-4A | TOR4A
- Origin Species
- Human
- NCBI GenBank
- NM_017723
- SwissProt
- Q9NXH8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 46.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDRGQPSLEP AAAAPRASGR CVIAPVRAVL RLRRRVCVLR KRRLLQPGGG PDVGTGAPRP61GCSPRAPRAD LDQPKFFTFD SPAELPSRTP RKKRRRSRLV LYPETSRKYR PRVEHRSRAQ121RCLLLLVAIV GFQVLNAIEN LDDNAQRYDL DGLEKALQRA VFGQPAAVSR IVALMRDYLA181THVHSRPLLL ALHGPSGVGK SHVGRLLARH FRSVLEDSAL VLQYHARHHC PEARAAQDCR241EELARRVADV VARAEAEEKT PLLVLDDVEL MPRPLLDELH GFLQPQRSHH FHNAIYVLLS301GAGGAEVTRF VLQNASRALP LRPDGFRSAE AAAAQAEEDL RASLLAVLSR EHPLWQAAAI361VPFLLLDKRD VVSCFRDEMA GEGFFPDQAR AENLAAQLSF YRVAGREFAV TGCKQVVATV421NLLSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
423 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.