Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TOR1AIP1 / LAP1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113119-100
Catalog Number: LS-G113119
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human TOR1AIP1 (NM_015602), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- LAP1B | Lamin-associated protein 1B | LAP1 | TOR1AIP1 | Torsin A interacting protein 1
- Origin Species
- Human
- SwissProt
- Q5JTV8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 66.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAGDGRRAEA VREGWGVYVT PRAPIREGRG RLAPQNGGSS DAPAYRTPPS RQGRREVRFS61DEPPEVYGDF EPLVAKERSP VGKRTRLEEF RSDSAKEEVR ESAYYLRSRQ RRQPRPQETE121EMKTRRTTRL QQQHSEQPPL QPSPVMTRRG LRDSHSSEED EASSQTDLSQ TISKKTVRSI181QEAPVSEDLV IRLRRPPLRY PRYEATSVQQ KVNFSEEGET EEDDQDSSHS SVTTVKARSR241DSDESGDKTT RSSSQYIESF WQSSQSQNFT AHDKQPSVLS SGYQKTPQEW APQTARIRTR301MQNDSILKSE LGNQSPSTSS RQVTGQPQNA SFVKRNRWWL LPLIAALASG SFWFFSTPEV361ETTAVQEFQN QMNQLKNKYQ GQDEKLWKRS QTFLEKHLNS SHPRSQPAIL LLTAARDAEE421ALRCLSEQIA DAYSSFRSVR AIRIDGTDKA TQDSDTVKLE VDQELSNGFK NGQNAAVVHR481FESFPAGSTL IFYKYCDHEN AAFKDVALVL TVLLEEETLG TSLGLKEVEE KVRDFLKVKF541TNSNTPNSYN HMDPDKLNGL WSRISHLVLP VQPENALKRG ICLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
583 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.