Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
TOR1A / Torsin A Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G98919-100
Catalog Number: LS-G98919
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Torsin A(TOR1A) (NM_000113), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DYT1 | TOR1A | Torsin A | DQ2 | Dystonia 1 protein | Torsin family 1 member A | Torsin-1A
- NCBI GenBank
- NM_000113
- SwissProt
- O14656
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 37.6 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MKLGRAVLGL LLLAPSVVQA VEPISLGLAL AGVLTGYIYP RLYCLFAECC GQKRSLSREA61LQKDLDDNLF GQHLAKKIIL NAVFGFINNP KPKKPLTLSL HGWTGTGKNF VSKIIAENIY121EGGLNSDYVH LFVATLHFPH ASNITLYKDQ LQLWIRGNVS ACARSIFIFD EMDKMHAGLI181DAIKPFLDYY DLVDGVSYQK AMFIFLSNAG AERITDVALD FWRSGKQRED IKLKDIEHAL241SVSVFNNKNS GFWHSSLIHR NLIDYFVPFL PLEYKHLKMC IRVEMQSRGY EIDEDIVSRV301AEEMTFFPKE ERVFSDKGCK TVFTKLDYYY DDSGPTRTRR LEQKLISEED LAANDILDYK361DDDDKV
366 aa
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.