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TONSL Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

TONSL Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all TONSL antibodies →
$504.00each
Catalog Number: LS-G114208-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human NFKBIL2(TONSL) (NM_013432), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
I-kappa-B-related protein | IkappaBR | IKBR | NFKBIL2 | Tonsoku-like protein | TONSL
Origin Species
Human
NCBI GenBank
NM_013432
SwissProt
Q96HA7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
132.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRTRLYLNLG LTFESLQQTA LCNDYFRKSI FLAEQNHLYE DLFRARYNLG TIHWRAGQHS
61QAMRCLEGAR ECAHTMRKRF MESECCVVIA QVLQDLGDFL AAKRALKKAY RLGSQKPVQR
121AAICQNLQHV LAVVRLQQQL EEAEGRDPQG AMVICEQLGD LFSKAGDFPR AAEAYQKQLR
181FAELLDRPGA ERAIIHVSLA TTLGDMKDHH GAVRHYEEEL RLRSGNVLEE AKTWLNIALS
241REEAGDAYEL LAPCFQKALS CAQQAQRPQL QRQVLQHLHT VQLRLQPQEA PETETRLREL
301SVAEDEDEEE EAEEAAATAE SEALEAGEVE LSEGEDDTDG LTPQLEEDEE LQGHLGRRKG
361SKWNRRNDMG ETLLHRACIE GQLRRVQDLV RQGHPLNPRD YCGWTPLHEA CNYGHLEIVR
421FLLDHGAAVD DPGGQGCEGI TPLHDALNCG HFEVAELLLE RGASVTLRTR KGLSPLETLQ
481QWVKLYRRDL DLETRQKARA MEMLLQAAAS GQDPHSSQAF HTPSSLLFDP ETSPPLSPCP
541EPPSNSTRLP EASQVHVRVS PGQAAPAMAR PRRSRHGPAS SSSSSEGEDS AGPARPSQKR
601PRCSATAQRV AAWTPGPASN REAATASTSR AAYQAAIRGV GSAQSRLGPG PPRGHSKALA
661PQAALIPEEE CLAGDWLELD MPLTRSRRPR PRGTGDNRRP SSTSGSDSEE SRPRARAKQV
721RLTCMQSCSA PVNAGPSSLA SEPPGSPSTP RVSEPSGDSS AAGQPLGPAP PPPIRVRVQV
781QDHLFLIPVP HSSDTHSVAW LAEQAAQRYY QTCGLLPRLT LRKEGALLAP QDLIPDVLQS
841NDEVLAEVTS WDLPPLTDRY RRACQSLGQG EHQQVLQAVE LQGLGLSFSA CSLALDQAQL
901TPLLRALKLH TALRELRLAG NRLGDKCVAE LVAALGTMPS LALLDLSSNH LGPEGLRQLA
961MGLPGQATLQ SLEELDLSMN PLGDGCGQSL ASLLHACPLL STLRLQACGF GPSFFLSHQT
1021ALGSAFQDAE HLKTLSLSYN ALGAPALART LQSLPAGTLL HLELSSVAAG KGDSDLMEPV
1081FRYLAKEGCA LAHLTLSANH LGDKAVRDLC RCLSLCPSLI SLDLSANPEI SCASLEELLS
1141TLQKRPQGLS FLGLSGCAVQ GPLGLGLWDK IAAQLRELQL CSRRLCAEDR DALRQLQPSR
1201PGPGECTLDH GSKLFFRRL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1219 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.